Association between growth hormone gene polymorphisms and growth traits in Cyprinus carpio from the Caspian Sea

Authors

  • Ayoub Farhadi Laboratory for Molecular Genetics and Animal Biotechnology, Faculty of Animal Sciences and Fisheries , Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
  • Ghodrat Rahimi Mianji Laboratory for Molecular Genetics and Animal Biotechnology, Faculty of Animal Sciences and Fisheries , Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
  • MohammadKazem Khalesi Department of Fisheries, Faculty of Animal Science and Fisheries , Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
  • Najmeh Berenjkar Department of Fisheries, Faculty of Animal Science and Fisheries , Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
Abstract:

Growth hormone (GH) is the most important hormone that controls somatic cell growth and the syntheses of protein, fat, and carbohydrates. This study was to identify gene polymorphism of GH-1 using PCR- single strand conformation polymorphism (SSCP) technique and its association with growth traits including condition factor (CF), body weight (BW) and total length (TL) in common carp (Cyprinus carpio). A total of 150 carp at 4 age classes of 4, 6, 12 and 24 months were randomly selected and DNA was extracted from caudal fin by modified salting out method. A fragment of 373 bp from exon 4, intron 4, and exon 5 of GH-1 gene was amplified using polymerase chain reaction (PCR). The genotyped samples by SSCP analysis yielded 8 different banding patterns as A, B, C, D, E, F, G and H with frequencies of 31.33, 10.67, 20.67, 22.67, 4.0, 2.0, 2.67 and 6.0 %, respectively. Analysis of marker-trait correlation by General Linear Model (GLM) showed significant associations between carp's GH-1 banding patterns and body weight, but the gene was not significantly associated with TL and CF parameters. The body weights of with a banding pattern of D were significantly (P

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Journal title

volume 17  issue 3

pages  533- 541

publication date 2018-07-01

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